Maximizing sample quality
The ability to obtain rake samples quickly without altering cells in the sample distribution is essential to maximize diagnostic quality. Syringe, vacutainer, and phonograph needle size should be appropriate for the size of the patient. Syringes that are besides big can damage cells and vessel walls with excessive vacuum. Roach reminds technicians to fill tubes appropriately, particularly lavender top tubes, which contain EDTA, as an inappropriate proportion of EDTA to sample can alter cellular telephone morphology. The clock of sample collection is besides important to maximize accurate results. Cell degradation can begin equally soon as the sample is collected. additionally, administration of fluids and medications can alter results, so samples should be drawn from patients anterior to administration of fluids and medications when possible. Patients who have eaten recently may have lipemic samples, which can interfere with some test results. Consider recommending a fast anterior to scheduled blood draw when scheduling appointments. When preparing a site for venipuncture, ensure that the skin is free from obvious infection and ignition. Roach notes that wiping the locate with alcohol prior to venipuncture not only removes superficial contamination but besides improves visual image by wetting down the fur and causing vasodilation of the vein beneath the surface of the skin. Full sterile preps should be performed if sampling is being done for blood cultures.
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finally, patient fear, tension, agitation, and clamber can alter some values, such as flannel blood cells ( i, presence of a try leukogram ), hydrocortisone, and lineage glucose ( specially in cats ). Repeated sampling from the lapp locate can besides increase leukocytes and platelets in the area, which could confound results. If a technician notes that any of these events are occurring, they should note it on the patient chart and inform the veterinarian in consign of the font to help aid in the most accurate interpretation of results .
There are numerous sites available for venipuncture in canine and feline patients. The most park sites are the cephalic and jugular vein veins, the lateral pass saphenous vein in dogs, and median saphenous vein in cats. For patients who may be hospitalized, the cephalic vein should be avoided for venipuncture as it is the choose side for placement of an intravenous catheter. Roach reminds technicians that proper restraint is critical for successful venesection. The animal trainer is creditworthy for positioning the patient in a way that maximizes visual image of the vein while maintaining affected role and staff base hit and minimizing affected role stress. If a vein can not be palpated or visualized, Roach does not recommend poking blindly as it is “ doomed to bankruptcy and causes unnecessary discomfort to the patient. ” Following sample distribution collection, pressure should be applied to the site for a minimal of 30 seconds. For patients with know coagulopathies, extra imperativeness will be needed .